BIOL 311 Lecture Notes - Lecture 6: Scanning Tunneling Microscope, Ficoll, Confocal Microscopy
17 Sep 2016
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Add detergent we end up with a mixed micelle - consists of molecules and proteins run through a dialysis system. 09/13 - lecture 6: sem - allows you to look at surface of cell, laser capture microdissection - allows you to pick out/ remove a cell. 7 microns ->30 allow to pick out cell and analyze it ie. cancer stem cells. Lower photoxicity (because of lower heat generated) compared to confocal microscopy in vivo applications in real time thicker images diffraction limited = abbes equation: super resolution microscopy. Not diffraction limited there are several types (see text) increasing our resolution (new ppt)methods - cell separation, fractionation and protein separation. Take tissue which is held tightly together, dissociate a single cell. To do this, must have two different components in a solution. 1. must have egta = ca+2 chelator calcium dependent cell adhesion molecules so remove calcium. 2. must have protease - hydrolyzes proteins.
