BIOL 401 Lecture Notes - Lecture 18: Zebrafish, Biosensor, Transgene
20 Apr 2017
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Lecture 18 animal models: elegans: non-parasitic nematode, small, easy to rear, consumes e. coli, and have a short lifespan, entire development has been mapped (959 cells in hermaphrodite, 1031 cells in males). Transparent so it"s easy to view cells: haploid number is 6, and consists of a 97 million base pair genome, hibernation state helps in cancer research. Zebrafish: small tropical vertebrae fish: hundreds of known mutants, transparent embryos. Makes it easy to snip a gene of interest and stick into the genome. For example, if we want to make a mutant fish wild- type again we can knock out the mutant gene and put in the wild-type gene: biosensor systems (slide 4). Known promoter is induced by estrogen and placed into zebrafish as a transgenic organism. We can put these fish into the water and see if certain chemicals/hormones are in the water. If these chemicals are present the fish will fluoresce.
