CAS BI 525 Lecture Notes - Lecture 29: Western Blot, Insulin, Synaptic Plasticity
17 Dec 2016
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Document Summary
4 proteins make gamma secretase: presenilin: 1 of the proteins in gamma secretase, makes the cut in app, which presenilin 1 (ps1) carries a high number of mutations familial. More than 70 mutations on presenilin associated with familial. Two forms of abeta peptide produced: 42 is more aggressive because there are more hydrophobic residues. This changes how it can interact with other peptides: 40. Abeta 42/ abeta 40: the higher the ratio the more aggressive: abeta 40 will always be greater, may signal for increase in amyloidogenic properties. What it toxic: beta amyloid plaques, oligomers. Abeta is the core of the plaque because they have the hydrophobic residues. More abeta 42 and/or aggregated abeta the faster the aggregation occurs into plaques. A(cid:373)yloid: (cid:374)ot (cid:894)a(cid:271)eta spe(cid:272)ifi(cid:272) to alzhei(cid:373)er"s(cid:895: congo red: identifies amyloid but not tau. Insolubility in water: beta-sheet conformation, largest aggregates deposit extracellularly, positive to staining with specific dyes (congo red, associated with tissue degeneration.
