CAS BI 525 Lecture Notes - Lecture 18: Cytochrome C, Transfection, Motor Neuron
31 Oct 2016
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Als & sod1: misfolding in apoptosis and transport (part 3: wt and mutant sod1 share an aberrant conformation and a common pathogenic pathway in. Sdg6: cannot detect mutation but detects sod1 (used for control) there is sod1 there: slide 29: clear change in histology, detecting misfolded protein and see it accumulating in sporadic patients. I: fals, sod(-) without mutation of sod1 disease is caused by something else: slide 30: is it toxic, mutant sod1 can interact with dynactin, axon of squid; cultured cells, this is when they make the mutation. One mutation: h46r, sod1 ox; wt sod1: make cell lysate and then put it on axon of squid, show how far the vesicles can go by staining the vesicles. In wt: kinetic of transport and distance did not change: kinetic would slow down with h46r (familial sod1) or sod1ox, evidence, oxsod1 reduces transport, conclusion: Bcl-2 exposing more bh3 domain: looks like it could be a cause.
