BIOL 1300 Lecture Notes - Lecture 3: Size-Exclusion Chromatography, Elution, Molar Mass
Document Summary
Often used in combination with size exclusion chromatography (shown as continuous trace and x-axis elution time) to show the malls signal (dots and y- axis) for individual components in a mixture. The y-axis isn"t for the sec, it"s for the malls. In sec, big particles elute faster than smaller particles. Scattering is more sensitive for bigger particles (proportional to the third power) Gives you direct information about molar mass of compounds. Works best for single species at a time, otherwise the signals from the big particles will obscure the smaller ones. Sample flows along path, multiple beams of light at different angles shine on it, extrapolate to value when angle = 0. Unclear what will be able to survive the ionization protocol of nmr, for example dimer interactions will be disrupted. Tells you mw of entire molecule, not charged states. **gold standard for determining mw, coupling with sec allows you to confidently separate species (self-consistency)
