BIOL 1300 Lecture Notes - Lecture 8: Dosage Compensation, Allosome, Immunoprecipitation

51 views3 pages
Department
Course
Professor

Document Summary

Immunoprecipitation: protein binds to coupled antibody and is purified from everything else in the lysate, also get all the proteins that bind to that protein (co-immunoprecipitation) Chip: enrich for dna sequences that bind a specific protein, typically a transcription factor (dna binding: *allows you to figure out what locations on the dna does the protein bind to, process. Cross-link protein to dna with uv or chemical (formaldehyde) Extract dna and shear/break into smaller parts by sonication. Bind protein with a specific antibody and precipitate with beads that bind to antibody. Chip-seq/chip: pair chip with sequencing techniques: sequencing or microarrays can be used to read out the dna sequencing. Targeting drosophila dosage compensation to the x chromosome. Proper gene dose regulation is essential for viability: complexes require proper stoichiometry, an imbalance in gene dose is associated with disease. Turner syndrome - deletion of one female x. Williams syndrome - deletion of one chromosome 7.

Get access

Grade+
$40 USD/m
Billed monthly
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
10 Verified Answers
Class+
$30 USD/m
Billed monthly
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
7 Verified Answers

Related Documents