BIOL 1300 Lecture 5: Antibiotics and the Ribosome
Document Summary
Each functional sub-unit is made of rrna and proteins. Process of translation: activation of aas. The ribosome is highly conserved --> makes it highly targeted by antibiotics. Various parts of the cycle can be targeted. Based on preventing or interrupting interaction between ribosome and antibiotic. X-ray crystallography: can solve high-resolution structure of complexes of molecules by looking at diffraction patterns of crystals. Measures position and presence of exon junction complex deposited on mrnas during splicing. Presence of stable complexes: stable enough to withstand displacement of transcribing reverse transcriptase. Foot-printing (rrna: look at susceptibility of ribosomal rna to chemical modification, covalently modify rrna, see at which point it gets modified, readout: gels of different lengths of rna, show where the chemical modification terminates reverse transcriptase. Fret (fluorescence/forster resonance energy transfer)/smfret (single molecule fret: ex. When close together, fret efficiency is high; when far apart, fret efficiency is low. C and e - impaired fret with drug.
