BIOS 12114 Lecture 8: 10-12 Macronutrients-HFCS, Carbohydrate Fiber, Alcohol
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How could we determine if the MH1 plasmid carries the ampicillin resistance gene?
A) Look at the plasmid on a gel |
B) | Sequence the plasmid DNA |
C) | See if it can transform normal E. coli |
D) | See if the plasmid digests ampicillin |
What procedure might the BEST way to find out an MH1 gene is related to any known genes from other organisms?
A) Compare multiple PCR markers across species |
B) | Sequence the DNA from the gene and use a BLAST search |
C) | Transform different bacteria and grow them on different antibiotics |
D) | Miniprep a plasmid and use a restriction digest |
Using the table below, explain the purpose of each of the steps of the Experimental Flow Diagram (Figure 1 from your lab manual) for answering the two research questions guiding this laboratory investigation:
Is the bacterial contamination at the three farms due to the same plasmid or different plasmids?
What is the frequency of antibiotic resistant bacteria in the farm cultures?
Box | Why are you doing this step? | How is this step relevant to research question(s) 1 and/or 2? |
Week 1: Experiment Part A | ||
Week 2: Experiment Part B | ||
Week 2: Experiment Part C | ||
Week 2: Experiment Part D | ||
Week 3: Calculate frequency of antibiotic resistance at farms | ||
Week 3: Use size markers on gel to determine size of resistance genes | ||
Week 3: Compare size to known size of resistance genes to identify which gene was present in each farm |