AVS-3100 Lecture Notes - Lecture 12: Immunodiffusion, Hemagglutination, Titer
2 Mar 2017
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Document Summary
Antibodies can be used a diagnostic tools. Taking blood can be to recover plasma or the serum. Heparin is used in medical purposes and is harvested from the small intestine of swine, it is an anticoagulant. For most purposes only the serum is needed, the blood clot forms and a centrifuge is not needed. Ab hemagglutination: different serum samples are diluted over and over in a log2 or two fold dilutions to linearize the dilution scheme, a titer is the antibody amount. This works by making sheep red blood cells the antigen and making antibodies for it still capable of cross linking and binding then determining what dilution is. Ouchterlony double-immunization: inner well has antigen and different antibodies are put in outer wells then it is observed for heavy precipitate lines, can be used to determine classes of antibodies. Immunoelectrophoresis: allows you to identify speci c proteins the antibody response is against.
