BIOL-1030 Lecture Notes - Lecture 12: Somatic Cell Nuclear Transfer, Hematopoietic Stem Cell, Recombinant Dna

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14 Nov 2016
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Adult stem cells and cord blood stem cells. Dna technology: a set of methods for the study & manipulation of an orga(cid:374)is(cid:373)"s dna, early approaches to gene identification. A gene that has been isolated from one organism, copied many times and stored in the genome of another organism. Gene can be retrieved, reproduced, transferred or expressed at any time. Dna) cut with the same enzyme: foreign dna anneal with plasmid dna via complementary base pairing, loose ends are joined with dna ligase to yield recombinant plasmid dna. Agrobacterium tumefaciens: an important pathogen of plants, promotes cancerous growth by transforming plant cells, derives nourishment by forcing plant growth. Engineering insect resistance in plants: important issues, crop losses due to insect damage range from 5% in wheat to 50% in cotton, chemical insecticides are expensive, non-specific, persistent & often need multiple applications.

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