MICR-4070 Lecture Notes - Lecture 9: Flow Cytometry, Immunoassay, Chromatography
13 Feb 2018
School
Department
Course
Professor
Document Summary
Quanititating components or structural features of cells primarily by optical means including light scatter and fluorescence intensities. Measuring one cell at a time & processing thousands of cells in a few seconds. Able to detect antigens, dna, mrna, & any structure with available antibodies. Able to count cells of different types. Works by: purifying a blob to get the specific components, the sheath fluid will go through the nozzle/channel and the laser will heat each cell. It scatters light which allows the size of each cell to be detected: it can also detect if a spore is present. Advantages: these aren"t dependent on the growth of microorganisms and are rapid/quick to do: this makes it good for microorganisms that take a long time to grow. Disadvantages: very sensitive so they may not give equivalent results to the culture methods and can be destructive to the microorganism. Enzyme linked immunoabsorbent assay (elisa: utilizes enzymes as a signal.
