BC 351 Lecture Notes - Phosphofructokinase 2, Conformational Change, Gluconeogenesis

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5 Aug 2014
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Glucose has 4 different thermodynamically favorable pathways in the liver cell. Regulation must occur for it to follow needed pathways at needed times. If both directions occur at the same time, nothing is created and atp is burned. Glucose consumed by yeast without and with o2 measured as fermentation occurred. Anaerobic flask consumes more glucose (cannot run mitochondrial processes to create atp) Extracellular signals, gene transcription, mrna degradation and/or translation. Allosteric enzymes are enzymes that undergo a conformational change that results in an increase or decrease in activity. Instead of km, k0. 5 ([s] at which v0 = vmax. When the enzyme is modulated, the k0. 5 or vmax will change (or both) Something covalently attaches to an enzyme (by a different enzyme) A steady state is a non-equilibrium state of a system where components remain at constant concentration. Fixed concentrations determine the q for each reaction. Metabolic flux is the rate at which metabolites flow through a pathway.

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