Light microscopes, electron microscopes

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Life Science
LIFE 212
Farida Safadi- Chamberlain

10 October Light Microscopes Bright Field Background is brightly lit Objective lenses above specimen Condensor below specimen Phase contrast Special lenses (annular stop turret in our lab exercises) Background light is separated from the light diffraction pattern generated by the specimen Dark field Background light is dark Opaque disk inserted between light source and condenser Dissecting (or stereoscope) Large working distance Used for handling large specimens Inverted Objective lens below specimen Condensor above the specimen Used for tissue culture Differential Interference Contrast Separates polarized light into two beams The length of each optical path differs, which gives the appearance of a 3D relief Fluorescence Compound or inverted Mercury or xenon light source Mirrors + excitation + emission filters allow only emitted light of a specific wavelength to be seen Confocal A special type of fluorescence scope Software allows digital capture of image slices Multiple images across the vertical Z axis are captured and reassembled in 3D reconstruction Reduces background fluorescence from unfocused parts of the specimen Electron Microscopes Transmission Electron Uses high energy electron beam to bombard the specimen (~100 nM thick) with electrons Dense parts of the specimen do not allow electrons to pass through the
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