Light microscopy, Kohler illumination, kingdoms

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Life Science
LIFE 212
Farida Safadi- Chamberlain

17 October Principles of Light Microscopy White light source is focused by 3 sets of lenses; condenser, objective, and ocular Magnification is distinct from resolving power (resolution) Light microscopy magnifies up to 500X over that detectable by human vision Optical Magnification: the ratio of the apparent size of an object to its true size Magnification is limited by Lens resolution: the ability to resolve detail (i.e., 2 points that are a given distance apart). Resolution is limited by diffraction. Diffraction is influenced by lens quality (NA). The Kohler Illumination adjustment is critical to achieving the uniformity of light on the specimen for photomicrography Most common alignment procedure Aligns the optic lenses and the light source Provides the highest intensity and most uniform illumination for the optical system Uniformity of illumination prevents shadows, glare and inadequate contrast 6 Kingdoms Plantae (algae, plants) Animalia Fungi Protista Monera Eubacteria Archaebacteria Kingdom classifications are in flux, due to ongoing research in Phylo
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