LIFE 212 Lecture Notes - Cytoplasmic Streaming, Archaea, Protist
30 Mar 2014
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Document Summary
White light source is focused by 3 sets of lenses; condenser, objective, and ocular. Light microscopy magnifies up to 500x over that detectable by human vision. Optical magnification: the ratio of the apparent size of an object to its true size. Lens resolution: the ability to resolve detail (i. e. , 2 points that are a given distance apart). The kohler illumination adjustment is critical to achieving the uniformity of light on the specimen for photomicrography. Aligns the optic lenses and the light source. Provides the highest intensity and most uniform illumination for the optical system. Uniformity of illumination prevents shadows, glare and inadequate contrast. Kingdom classifications are in flux, due to ongoing research in phylogenomics. Visible features are limited by the imaging method used. Onion cells nucleus & vacuole (difficult to see) Compare features of fresh-mounted cheek cells to those of pre-mounted specimens. Give complete information about images for which observations are given (write complete photo caption in answer)
