Principles of Light Microscopy
White light source is focused by 3 sets of lenses; condenser, objective, and ocular
Magnification is distinct from resolving power (resolution)
Light microscopy magnifies up to 500X over that detectable by human vision
Optical Magnification: the ratio of the apparent size of an object to its true size
Magnification is limited by
Lens resolution: the ability to resolve detail (i.e., 2 points that are a given distance apart).
Resolution is limited by diffraction.
Diffraction is influenced by lens quality (NA).
The Kohler Illumination adjustment is critical to achieving the uniformity of light on the specimen
Most common alignment procedure
Aligns the optic lenses and the light source
Provides the highest intensity and most uniform illumination for the optical system
Uniformity of illumination prevents shadows, glare and inadequate contrast
Plantae (algae, plants)
Kingdom classifications are in flux, due to ongoing research in Phylo