LIFE 212 Lecture Notes - Immunoassay, Pipette, Antibody
30 Mar 2014
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Document Summary
Theory (or background information) of the use of elisa (enzyme-linked immunosorbent. A special type of protein protein interaction. Antibodies are a type of immune-response protein made in vertebrates. Is used in many applications in research labs. Antibodies are proteins that are produced as part of the adaptive immune response to potential pathogens foreign antigens. Abs are produced on the surface of white blood cells (b cells): Many types of ab molecules are produced at different times in the immune response. Antigens are defined as any foreign substance detected by the immune system. Each ab binds to a single ag binding site (epitope) Response to a single antigen results in many types of antibodies (polyclonal antibodies) Use an immunoassay to detect the antigen-antibody response. Specificity is based on the highly precise interaction between antibodies and their antigens (ab/ag) Sensitivity is based on the ability to detect a very few molecules in a complex mixture.
