MIP 300 Lecture Notes - Lipid Bilayer, Electron Microscope, Epitope
Document Summary
Components for synthesis of proteins and nucleic acids. May have more than 1 piece segmented genomes ssrna: + or strand, + =mrna, -= compliment ssdna: + or strand, - = template strand, + = non-template strand dsrna dsdna. Diameter of virion (virus particle outside of cell) ~10 300 nm. Immunogenic properties what antibodies attach to it (what epitopes it has) Intracellular location of replication cytoplasm (rna, except influenza) or nucleus (dna, except pox) Tissue culture cells growing in petri dishes. Some viruses cause cytopathic effects damage or death to cells which can be visualized and indicate viral infection. Due to small size can separate virus from cells by. 1st spin ~8,000 rpm removes large cells. Add virus isolated by differential centrifugation to sucrose density gradient. Spin virus will settle where sucrose density = viral density. Plaque assay look at cytopathic effect, count number of plaques. Hemagglutinin assays some viruses cause rbc"s to clump (agglutinate)