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Lecture

MIP 300 Lecture Notes - Eukaryotic Transcription, Cistron, Precursor Mrna


Department
Microbio, Immun, Pathology
Course Code
MIP 300
Professor
Erica Suchman

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27 March
Central Dogma of Molecular Biology
Flow of information: DNA → RNA (transcription) → protein (translation)
The structure of RNA
3 types
Ribosomal – rRNA makes up ribosomes
Transfer – tRNA carries amino acids to ribosome during translation
Messenger – mRNA, complimentary copy of template strand of DNA
Each gene has only 1 template strand: codes for mRNA
Either strand can be template
DNA transcription (mRNA synthesis in prokaryotes)
The substrates
4 nucleotides found in RNA. Bases added one at a time, energy is required to
add bases
[ATP, GTP, CTP, UTP] → RNA + nPPi
The participants
In bacteria: 1 RNA polymerase, transcribes all RNA, bases added 5’ to 3’ (DNA is
read 3’ to 5’)
RNA polymerase binds to a promoter region
-35 base pair before start site (+1) – RNA pol recognition site
-10 base pair before start site – Pribnow box (TATAAT), RNA pol binding
site, starts to unwind DNA
Transcription begins at +1
mRNA is polygenic – 1 mRNA → 1 protein
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