MIP 300 Lecture Notes - Eukaryotic Transcription, Cistron, Precursor Mrna

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lemondog142

1 Jul 2014

School

CSU

Department

Microbio, Immun, Pathology

Course

MIP 300

Professor

Erica Suchman

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Document Summary

Flow of information: dna rna (transcription) protein (translation) Transfer trna carries amino acids to ribosome during translation. Messenger mrna, complimentary copy of template strand of dna. Each gene has only 1 template strand: codes for mrna. Bases added one at a time, energy is required to add bases. [atp, gtp, ctp, utp] rna + nppi. In bacteria: 1 rna polymerase, transcribes all rna, bases added 5" to 3" (dna is read 3" to 5") 35 base pair before start site (+1) rna pol recognition site. 10 base pair before start site pribnow box (tataat), rna pol binding site, starts to unwind dna. Transcription begins at +1 mrna is polygenic 1 mrna 1 protein. Termination usually signaled by presence of rna hair pin loop. Large precursor rna made in nucleus called hnrna (heterogenous rna) hnrna is subject to post-transcriptional modification processing (splicing) Allows eukaryotes to produce more than 1 mrna/protein from a piece of dna by differential splicing.

Related Questions

RNA has many different roles in gene expression. Check all thatapply.

mRNA defines the polypeptide to be formed

DNA defines the polypeptide to be formed

rRNA, along with proteins, forms the ribosome

mRNA delivers the appropriate amino acid to the ribosome

tRNA delivers the appropriate amino acid to the ribosome

Translation is appropriately named because the process transfersthe code from DNA to RNA, though in complementary fashion.

True

False

The template strand is to the sense strand as the coding strandis to the antisense strand.

True

False

The phenomenon symbolized by DNA â RNA â Protein is referred toas the Central Dogma because it is true for all organisms.

True

False

The reading frame refers to the selection of the correct tripletof nucleotides by selecting the correct beginning point. If thebeginning point is wrong, all nucleotides will be clustered intothe wrong codons.

True

False

Activating enzymes need to recognize only one anticodon.

True

False

coffeehorse275

If the bold term in each statement is correct hen write the letter C next to it. if the term is incorrect then replace it with the correct term.

The nucleotide bases found only in RNA and not in DNA is thymine.

The class of RNA molecules that carries the amino acids that are added to the growing polypeptide chain is tRNA.

During translation immediately after the first and second tRNA molecules become attached side by side on the mRNA., a hydrogen bond is formed between adjacent amino acids.

In the genetic code, 20 bases on the DNA code for a single amino acid.

The major lipid involved is transcription is RNA polymerase.

The one strand of DNA used as a template in the making of mRNA during transcription, is the template strand.

chocolatemuskrat416

1. A region of a chromosome contains two strands of DNA, yet only one is used to transcribe a gene because

A. all genes are found on the same strand of DNA in a chromosome.

B.both strands are transcribed, but only one strand of DNA contains a reading frame.

C.RNA polymerase binds to the terminator, stopping transcription on one strand of DNA.

D.RNA polymerase binds to the promoter, which precedes the gene only on the template strand of DNA.

E.the start of transcription is random along the chromosome and doesn't always occur on a gene.

2. In eukaryotes an mRNA molecule can only be translated by one ribosome at a time.

True or False

3.The process by which cells use the information of RNA molecules to make proteins is transcription.

True or False

MIP 300 Lecture Notes - Eukaryotic Transcription, Cistron, Precursor Mrna

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