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SOCR 330 (39)
Sarah Ward (39)
Lecture

Prokaryote vs. eukaroyote transcription, introns

2 Pages
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Department
Soil + Crop Sciences
Course Code
SOCR 330
Professor
Sarah Ward

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19 October
Prokaryote vs Eukaryote Transcription
Prokaryote Eukaryote
Promoter consensus sequences TATAAT (-10)
and TTGACA (-35)
Promoter consensus sequences TATA (-25)
and CAAT (-60 to -120). Plants have AGGA
instead of CAAT.
No enhancers Enhancers often present
One RNA polymerase with alpha subunit
makes all RNA types
RNA pol I → rRNA
RNA pol II → mRNA
RNA pol III → ssRNA, tRNA
mRNA is primary transcript (“ready to go”)
Primary transcript is heterogenous mRNA
cannot be transcribed directly. Requires
processing before translation
Sigma subunit of RNA polymerase binds
directly to promoter
RNA pol II cannot bind directly to promoter –
requires protein transcription factors to bind to
DNA promoter region first
Termination may involve rho factor protein No rho factor protein for termination
Closely-coupled transcription-translation
possible
Transcription and translation always separate
events
Splicing Out Introns
Some RNA self-excise (autocatalytic RNAs) using ribozymes
Most RNAs require formation of spliceosome to remove introns
Spliceosome contains snRNPs (small nuclear ribonucleoproteins)
Cut at 5’ splice site first then form loop (“lariat”) to cut at 3’ site
Processing Heterogenous Nuclear RNA in Eukaryotes
Post-transcriptional modification of RNA transcripts in eukaryotes includes

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Description
19 October Prokaryote vs Eukaryote Transcription Prokaryote Eukaryote Promoter consensus sequences TATA (-25) Promoter consensus sequences TATAAT (-10) and TTGACA (-35) and CAAT (-60 to -120). Plants have AGGA instead of CAAT. No enhancers Enhancers often present RNA pol I → rRNA One RNA polymerase with alpha subunit makes all RNA types RNA pol II → mRNA RNA pol III → ssRNA, tRNA Primary transcript is heterogenous mRNA – mRNA is primary transcript (“ready to go”) cannot be transcribed directly. Requires processing before translation Sigm
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