SOCR 330 Lecture Notes - Uracil, Consensus Sequence, Noncoding Dna
Document Summary
Production of mrna copy (transcript) of the sequence on the dna coding strand of a gene. Transcript carries coding information out into cytoplasm where it acts as template for assembly of polypeptide. Rna transcript is complementary copy of dna template strand. Transcript is same (5" 3") as coding dna strand except has uracil (u) instead of thymine (t) Recognition sequences to guide rna polymerase into position. 10 and -35 boxes contain consensus sequences (conserved among different species) Up mutation brings promoter closer to consensus sequence and strengthens it. Down mutation makes promoter less like consensus sequence and weakens it. Promoter region base sequences in e. coli genes. The more closely the sequences at -10 and -35 resemble the consensus sequence the stronger the promoter will be. Single-stranded (ss) non-coding dna to act as template. All 4 rna triphosphate nucleotides (building blocks for mrna) Rna polymerase (enzyme to join nucleotides together to make mrna)
