Initiation of translation, elongation, termination of translation

2 Pages

Soil + Crop Sciences
Course Code
SOCR 330
Sarah Ward

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24 October Initiation of Translation in Prokaryotes Part I 30S subunit binds to Shine-Dalgarno sequence (AGGAGG) in mRNA upstream of start codon 16S rRNA within 30S subunit has sequence CCUCC at 3’ end Forms initiation complex with IF (“initiation factor”) proteins and GTP Initiation of Translation in Prokaryotes Part II Formylmethionine (fMet) placed on start codon within initiation complex IF3 released, 50S subunit binds Remaining IF proteins released, GTP hydrolyzed fMet tRNA bound to mRNA in P site, A site vacant for next charged tRNA Formylmethionine (fMet) Has COH (formyl) group added Usually positioned as first amino acid in polypeptide chain as translation starts in prokaryotes Almost always subsequently removed from final protein product Initiation of Translation in Eukaryotes
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