Sources of induced mutation

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Department
Soil + Crop Sciences
Course
SOCR 330
Professor
Sarah Ward
Semester
Fall

Description
9 November Sources of Induced Mutation Chemical mutagens Base analogs (e.g. 5-BU, 5-bromouracil) Alkylating agents (e.g. EMS : ethylmethane sulfonate) Acridine dyes (e.g. proflavin) Ionizing radiation UV light X rays, gamma rays, cosmic rays 5-Bromouracil (5-BU) Base analog that resembles thymine Incorporated into new DNA during replication A-Bu pair forms at normal AT site 5-BU tends to undergo tautomeric shift from keto to enol form Now pairs with guanine and induces base substitution when DNA replicates Ethylmethane Sulfonate (EMS) Adds alkyl (C2H 5 group to guanine 6-ethylguanine now pairs with thymine Induces base substitutions Proflavin Acridine dye Intercalates between bases
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