Gene regulation, lactose metabolism in E. coli

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Colorado State University
Soil + Crop Sciences
SOCR 330
Sarah Ward

28 October Gene Regulation All cells contain entire genome but not all genes are expressed at all times “Housekeeping” genes maintain general cell functions Expressed at high level in all cells Other genes have more specialized functions and are expressed Only in some cells Only under some conditions Gene Regulation Co-ordinate regulation (prokaryotes) One promoter for several genes Each gene – cistron Whole group of genes – operon Autoregulation (“feedback”) Gene controlled by its own product Negative regulation Inhibitor on promoter Inducer binds to and removes inhibitor Positive regulation Effector binds to promoter Jacob and Monod French researchers Awarded Nobel Prize for medicine in 1965 for their work on the lac operon Lactose Metabolism in E. coli Preferred sugar for ATP production is glucose (monosaccharide) Can use lactose if necessary Less efficient as lactose is dissacharide Must be cleaved and converted to glucose Slower to diffuse into cell E. coli and Lactose To utilize lactose as energy source E. coli needs: Beta-galacto
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