EBIO 1210 Lecture Notes - Lecture 13: Telomerase, Dna Ligase, Primase

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Molecular basis for inheritance: dna replication: search for the genetic material, griffith (1928): discovered transformation, which is the assimilation of external. They concluded dna was the transforming substance: hershey and chase (1952): used bacteriophages radioactively labeled with phosphorus and sulfur to demonstrate that dna was the genetic material. Their experiments showed that radioactive labeled phosphorus entered the bacteria, while the radioactive labeled sulfur did not. 5): chargaff observed that n-bases in dna molecules are found in fixed ratios (a=t; c=g) in each species. This is called chargaff"s rules: watson and crick (1953): armed with the information above and by using the x- ray photographs of dna of rosalind franklin (fig. 6), watson and crick proposed that the structure of dna was a double helix. Dna replication is the reproduction of the dna molecule: models for dna replication (fig. 12-18: origins of replication: 1 for bacteria; many for eukaryotes: helicases bind to origin of replication site and separates the dna strands.

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