LSP 121 Lecture 1: Chapter 3: Functions
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microbiology double unknown
1. G+ coccus
*tiny colonies
*white colony
*sediment
*Phenol Red:
PR Glu: (A/-) Yellow
PR Lac: (A/-) Yellow
PR Suc: (A/-) Yellow
*SIM test
Sulfure reduction: (-) No black
Indol: (-) No pink
Motility: (+)
*MR: (+) Red
*VP: (+) Red (redish brown)
* Red blood agar: beta hemo
*MacConkey: (-) No growth (G+)
*DNAse: (+) clearing around
*Gelatin: (+) liquid in 5 days
*Nitrate reduction: (+) red when added 2 indicators
*Citrate: (-) green
*Casein: (-) unclear
* EMB: (-) no growth
*MSA: (+) with yellow halo
*starch: (-) no clear around
2. Gram - bacillus
*tiny colonies
*yellow and sometimes red color inside of colony
*colony forming unit: Flocculent
*oxidase: (-) no blue
*Phenol Red
PR Glu: (A/-) Yellow
PR Lac: (-/-) No color change
PR Suc: (A/-) Yellow
*SIM test
Sulfur reducation: (-) No black
Indole: (-) no pink
motility: [+] little growth inside
*MR: (-) No color change
*VP: (+) red (red on the top and yellow on the bottom)
*Red blood cell Agar: alpha hemo
*MacConkey: G- and no color change (No fermentt lactose/noncoliform)
*DNAse: (+) clearing around
*Gelatin: (+) in 1 day
*Nitrate: (+) red after adding indicators
*Citrate: (+) blue
*Casein: (+) clear ........... (NOT SURE)
*EMB: poor growth
*MSA: (+) with yellow halo
*TSI: (A/A)(NG) yellow top and bottom, no gas
*Starch: (+) clear around after adding indicator
What is thename of Gram+ and Gram- Bacteria?
My instiructor gave us this bacteria list (he said someunknown bacteria can be not on the list)
B. Cereus |
E. coli |
E. aerogenes |
E. faecalis |
Streptococcus bovis |
K. pneumoniae (pneumoniae |
S. aureus |
S. epidermidis |
Moraxella Moratella Osloensis |
P. vulgaris |
B. subtilis |
M. luteus |
P. aeruginosa |
Bacillus Alvei |
S. marcescens |
A. faecalis |
Staphylococcus Saprophyticus |
Alcaligenes latus |
Morganella morganii |
S. sonnei |
C. xerosis |
Listeria innocua |
Proteus mirabilis |
Streptococcus agaloctiae |
Salmonella Choleraesais |
S. mitis |