BIO 330 Lecture Notes - Lecture 20: Focal Adhesion, Tight Junction, Hemidesmosome
BIO 330 Lecture notes 20:
• Focal adhesion
• links cell to ECM
• Adhesion belt
• belt that links one cell to another cell
• -membranes don't actually touch
• Desmosome
• Anchoring junctions that prevents cells subjected to mechanical stress from being pulled
apart; button like thickenings of adjacent plasma membranes connected by fine protein
filaments
• -bottleneck structure
• Hemidesmosome
• links cell to ECM
• Space between junctions (3)
• adhesive junctions = 20-35nm
• Tight junction = 0
• Gap Junction = 2-3nm
• Extracellular matrix (ECM) is the extracellular part of animal tissue that usually provides
___________ ___________ to the cells
• structural support
• 3 classes of ECM molecules
• structural proteins
• proteoglycans
• adhesive glycoproteins
• Structural proteins
• -class of ECM
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• -such as collagin and elastin that provide strength and flexibility
• Proteoglycans
• the protein-polysaccharide that provide the matrix
• Adhesive glycoproteins
• -fibronectins and laminins
• -allow components of ECM to connect to each other and allow cells to attach to the
matrix
• two methods in denaturing DNA
• raising the temperature (H bonds will become weak)
• put it in an alkaline solution
• the extent of denaturation of DNA can be monitored by the change in
______________________
• the light absorbing properties of DNA
• at what wavelength is the maximum absorbance in DNA
• 260nm
• DNA melting temperature (Tm)
• the temperature at which one-half of the absorbance change has been achieved
• what happens to the absorbance when the temperature of DNA solution is increased?
• the double helix meltsand the absorbance increases because single stranded DNA has
higher absorbance
• DNA melting temperature increases with its _________ content
• G-C content
• two ways DNA can be cut by restriction enzymes
• blunt ends
• sticky ends
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