BIO 330 Lecture Notes - Lecture 20: Focal Adhesion, Tight Junction, Hemidesmosome

44 views5 pages
24 May 2018
School
Department
Course
BIO 330 Lecture notes 20:
Focal adhesion
links cell to ECM
Adhesion belt
belt that links one cell to another cell
-membranes don't actually touch
Desmosome
Anchoring junctions that prevents cells subjected to mechanical stress from being pulled
apart; button like thickenings of adjacent plasma membranes connected by fine protein
filaments
-bottleneck structure
Hemidesmosome
links cell to ECM
Space between junctions (3)
adhesive junctions = 20-35nm
Tight junction = 0
Gap Junction = 2-3nm
Extracellular matrix (ECM) is the extracellular part of animal tissue that usually provides
___________ ___________ to the cells
structural support
3 classes of ECM molecules
structural proteins
proteoglycans
adhesive glycoproteins
Structural proteins
-class of ECM
find more resources at oneclass.com
find more resources at oneclass.com
Unlock document

This preview shows pages 1-2 of the document.
Unlock all 5 pages and 3 million more documents.

Already have an account? Log in
-such as collagin and elastin that provide strength and flexibility
Proteoglycans
the protein-polysaccharide that provide the matrix
Adhesive glycoproteins
-fibronectins and laminins
-allow components of ECM to connect to each other and allow cells to attach to the
matrix
two methods in denaturing DNA
raising the temperature (H bonds will become weak)
put it in an alkaline solution
the extent of denaturation of DNA can be monitored by the change in
______________________
the light absorbing properties of DNA
at what wavelength is the maximum absorbance in DNA
260nm
DNA melting temperature (Tm)
the temperature at which one-half of the absorbance change has been achieved
what happens to the absorbance when the temperature of DNA solution is increased?
the double helix meltsand the absorbance increases because single stranded DNA has
higher absorbance
DNA melting temperature increases with its _________ content
G-C content
two ways DNA can be cut by restriction enzymes
blunt ends
sticky ends
find more resources at oneclass.com
find more resources at oneclass.com
Unlock document

This preview shows pages 1-2 of the document.
Unlock all 5 pages and 3 million more documents.

Already have an account? Log in