MCB 2000 Lecture 9: Chapter 9 Microbial Genetics

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12 Oct 2017
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Research in genetics takes place on several levels. Genetic information is contained within genetic material - dna and rna. Chromosome - cellular structure - packaged dna molecule. Gene: a segment of dna that codes for functional product (protein) Genotype - the sum of all genes - genetic make-up. Phenotype: manifestation of genotype (ability to perform particular chemical reaction) Human cell: 25k - 30 k genes. Size of the cell of e. coli - 1 um (diameter. Size of the stretched out dna - 1 mm (1000 times longer) Dna molecule is tightly packed in the form of chromosome. Single strands of dna have opposite orientation (3" and 5") Synthesis of the new strand by attachment of complementary nucleotides. Helicase - untwists the dna helix by breaking the h bonds. Dna polymerase iii - adding bases to the new dna. Ligase - joining of the of dna fragments. Lagging strand is synthesized in pieces (okazaki fragments)

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