MCB 2000 Lecture Notes - Lecture 10: Pectin, Agarose Gel Electrophoresis, Restriction Enzyme
Document Summary
Recombinant dna technology - procedures by which a fragment of dna (gene) of one (food, antibiotics, vitamins) organism is incorporated into the genome of a different organism. Create organisms that synthesize products humans need . Introduce new genetic traits into an organism . (e. g. ) suppression of ripening in tomatoes) They recognize and cut specific fragments of dna (sequences of nucleotides in. Dna from different sources cut with the same restriction enzyme will produce the. Dna is cut on a specific palindromic sequence. Palindromes are sequences that are identical when read in opposite directions in same type of sticky end. In agarose gel, dna fragments are subjects to an electrical current. Dna molecule has a negative charge - moves toward the positive pole. A fragment of a single-stranded nucleic acid (dna, rna) can hybridize (unite) w/ Hybridization w/ a probe another fragment that has a complementary sequence of nucleotides sample by using a gene probe.
