PSB-2000 Lecture Notes - Lecture 5: Autoradiograph, Functional Magnetic Resonance Imaging, Green Fluorescent Protein
11 Oct 2017
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Document Summary
Based on the binding of labeled protein-specific antibodies. Expression of a receptor/protein depends on appropriate . Presence at the rna level does not mean the protein is guaranteed to translate. Minimally invasive and does not require euthanasia. Works at the transcription level to create crrnas. Dna genetically modified to express light sensitive ion channels. Other applications include memory disruption, aggression, and attraction. Green fluorescent protein exhibits bright green fluorescence when exposed to blue light. These gfp genes can be inserted into the dna of neurons-color can then be viewed. Fantastic fluorescence and the brainbow when targeted neuronal genes are expressed. Brainbow- developed by researched at harvard in 2007 avoidance. Morris water max- the rat must find a hidden platform in an opaque pool. 3d representation of the brain (structure only); x-ray (radiation) Scan is an image of level of radioactivity in varous parts. Measures waves emitted by hydrogen atoms activated within a magnetic field.
