ANAT 2130 Lecture Notes - Lecture 3: Inner Cell Mass, Ovulation, Artificial Insemination
16 Oct 2016
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Document Summary
About 10% of couples attempting conception fail. Male factors: low sperm count, poor sperm motility. Female factors: lack of ovulation, blocked uterine tubes, uterine septum. Sperm donation or egg donation is a way around genetic disease being passed on to offspring. If both parents are heterozygous carriers for recessive mutation, 25% of their offspring will be afflicted, and 50% will be carriers. Sperm or egg donation circumvents this problem: so does blastomere biopsy. Sample dna of one blastomere of 4 or 8 cell pre-embryo. Do genetic analysis of that dna after pcr (polymerase chain reaction) Determine which embryos of a group are homozygous wild type and implant only those embryo. Removal of early blastomeres still results in formation of complete fetus: blastomere biopsy possible for prenatal genetic manipulations. Dyes injected into one of four blastomeres end up in both trophoblast and embryoblast: therefore, early blastomeres are totipotent. Male infertility can be circumvented by sperm donation.
