HONR 1033 Lecture Notes - Lecture 6: Polymerase Chain Reaction, Plasmodium, Transcription Activator-Like Effector Nuclease
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1. important to be able to identify so that transmission of diseases can be slowed or halted. B. pcr - polymerase chain reaction, used to identify mutations for further utilization or amplify malaria) expression of a gene. Cycle 1 yields 2 molecules: 1. denaturation, 2. annealing, 3. extension. C. ngs; used to determine specific genotype (application of pcr) length. 3. using pcr, 10^6 copies of each fragment are made, each attached to the. 4. the bead is placed into a well with dna polymerases and primers. 5. solutions of each of the four nucleotides are added to all wells sequentially and. 6. if a nucleotide is joined to a growing strand, pp, is released, causing a flash of. 2. result is a flowchart with different peaks/bars for each nucleotide being represented. D. application of cloning; recombinant molecule- consists of two different dna (from separate. 1. possess circular plasmids: replicate on their own, are used by molecular biologists.
