Life Sciences 1a Lecture 14: Lecture 14 – Transcription.docx
10 Feb 2014
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5" methyl group irrelevant to base pairing uracil is recognized as foreign in dna and replaced with cytosine to prevent mutation effects if dna had uracil, cytosines that had undergone deamination could not be recognized doesn"t matter for rna because rna is not genetic material no lagging strand in transcription promoters tell rna polymerase where to start transcribing upstream of transcription start site (+1) refer to non template strand no individual promoter is perfect match for platonic ideal (cid:224) ttgaca most common in statistical analysis sigma contacts and recognizes 35 and 10 sequences, forms closed complex then open complex then initiation then elongation then termination eukaryotic rna polymerase not intrinsically capable of recognizing promoters transcription factors bind to promoter and recruit rna polymerase.
