Molecular and Cellular Biology MCB 52 Lecture Notes - Nucleic Acid Structure, Quinolone, Ciprofloxacin
17 Feb 2014
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Objectives: explain how the mathematical concept of linking number and two related geometric properties (twist and writhe) relate to the topology of dna molecules, describe the physiological consequences of maintaining dna in a supercoiled state, predict how the topology of dna molecules affects their mobility in an electrophoretic field and diagram how that was used to experimentally determine the helical periodicity of dna in solution. Objectives: define the following terms: chromosome, 30 nm fiber, nucleosome, histone, describe the composition and structure of the nucleosome, including, the stoichiometry of the histone core proteins, the significance of the positively charged amino acids that make up the histone proteins, how histone h1 affects the arrangement of nucleosomes on the dna, how the dna wraps around the nucleosome and its significance, explain how nucleases can be used to reveal how nucleosomes interact with dna. Leading s t r and s y n the si s.
