MCB 52, October 22, Section Notes.docx

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Department
Molecular and Cellular Biology
Course
Molecular and Cellular Biology MCB 52
Professor
Tom Torello
Semester
Fall

Description
IPTG (non­hydrolyzable), binds to the repressor and induces transcription >> preferentially induce transcription of proteins >> only when cells are in log phase of growth Hypothetically, protein will be in soluble fraction >> if bacteria produces too quickly, will go to inclusion bodies, in the liquid >> also, samples w/ and w/out SDS, SDS is the buffer that creates generable negative charge on buffer,  allows for Western blot to be conducted and proteins to be separated on the gel >> but for EMSA, we want protein in native form, so no SDS and stored in the ­80 to prevent them from  being degraded LAB REPORT No intro & discussion for this one Target PCR >> if figure with multiple lanes, all lanes have to be included; alternatively, if you crop image to only  inc
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