BIOL 212 Lecture Notes - Lecture 13: Reverse Transcriptase, Central Dogma Of Molecular Biology, Rna

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chocolateoyster191

26 Sep 2016

School

Iowa State University

Department

Biology

Course

BIOL 212

Professor

Kukday Sayali

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Document Summary

Dna (information storage) mrna (messanger rna) (information carrier) proteins (active cell machinery) Dna is found in the nucleus, protein synthesis takes place in the cytoplasm. An organism"s genotype is determined by the sequence of bases in its dna. An organism"s phenotype is a product of the proteins it produces. Alleles of the same gene differ in their dna sequence. Proteins produced by different alleles of the same gene frequently differ in their amino acid sequence. We have 23 pairs of chromosomes, 1 of these is for sex. These other rnas perform important functions in the cell. Ex) some viral genes are composed of rna; use reverse transcriptase, a viral polymerase to synthesize a dna version of the virus"s rna genes. Some amino acids are specified by more than one triplet code. Signifies the start of the protein-encoding sequences in mrna there are three stop codons (uga, uaa, and uag) All amino acids except two are encoded by morethan one codon.

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Acetyl CoA is the beginning of a metabolic pathway that produces most of the NADH and FADH for ATP production. When you starve, which of the following will produce the most Acetyl CoA?

Protein, carbohydrates, and lipid metabolism all produce the same amount

Free Oxygen radicals

Protein metabolism

Lipid metabolism

Carbohydrate metabolism

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Which of the following occurs when oxyhemoglobin reaches the capillaries of heart muscle?

Absorbs oxygen from the mitochondria

Releases oxygen which leaves the capillary and diffuses into the cell

Releases its oxygen which diffuses from the capillary to the nucleus

Releases its oxygen which diffuses from the mitochondria to the capillary

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The drug, cannabis, which is produced by the marijuana plant, is a byproduct of which reaction?

Dark reaction

Light reaction

Oxidative phosphorylation of plants

Glycolysis in plants

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All of the following are true about bile EXCEPT:

It acts as an emulsifying agent for lipids and it is synthesized in the liver

It disperses globs of fat in the chyme into microscopic particles

It acts as an emulsifying agent on carbohydrates and is synthesized in the gall bladder

It exposes the large surface area of lipids to lipase

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The carbon-nitrogen bond that connects amino acids together in a protein is referred to as which of the following?

Peptide bond

Covalent bond

Ionic bond

Octet bond

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Dr. Monroe wants to prevent Priscilla, the chair of biology, from digesting carbohydrates. Which organ should she remove from Priscilla that produces the enzymes needed for carbohydrate digestion?

Small intestine

Liver

Pancreas

Large intestine

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QuestionGroup: #17 Lipids

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Homework

All of the following are true about the liver EXCEPT:

It receives all the blood from the intestinal system by way of the portal circulation.

It produces iron directly with no need for having a food substrate

It stores fats and carbohydrates for energy.

It regulates blood glucose levels and synthesis of blood proteins

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QuestionGroup: #18 DNA

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This molecule controls the production of enzymes in animal cells.

It is DNA, a nucleotide that resides in the nucleus of cells

It is RNA, the ancestor to DNA

It is a protein

It is DNA, a carbohydrate

It is DNA, a nucleotide which resides in the cytoplasm of the cell

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QuestionGroup: #19 DNA2

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Homework

Which of the following represents the proper sequence for the "central dogma"?

RNA to Protein to DNA

RNA to DNA to Protein

DNA to RNA to Protein

Protein to RNA to DNA

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QuestionGroup: #20 Gene expression

Assigned as

Homework

How many different kind of amino acids are necessary for mRNA to make proteins in humans?

Bogus question since mRNA does not use amino acids to make protein.

Bogus question since mRNA does not use amino acids to make protein rather it is the rough endoplasmic reticulum.

13. The arms of a human and the wing of a bat are _________________ structures, composed of the same bones that have been modified for different functions.

analogous

vestigial

c.	homologous

d.	convergent

14. Xeroderma pigmentosum is an autosomal recessive genetic disorder. People who are homozygous for the disease-causing allele have skin that is damaged by exposure to ultraviolet radiation; this can lead to skin cancer at an early age. The mutation that causes this disorder is in a gene that functions:

a.	to repair DNA by excising (removing) nucleotides damaged by UV light.

b.	in lysosomes, to break down lipids that otherwise accumulate in the nervous system.

c.	to produce the UV blocking pigment melanin in the skin cells

d.	in red blood cells to carry oxygen to the skin.

15. The Amish are a religious sect descended from a small group of colonists who came to the United States over 200 years ago. Their religious beliefs have kept them isolated from society. The Amish have a much higher than average incidence of both polydactyly (extra fingers and toes) and dwarfism in their communities. This is most likely explained by:

a.	inheritance of acquired characters.

b.	the founder effect.

c.	disruptive selection.

d.	natural selection.

16. In the structure of DNA that Watson and Crick proposed, the sides of the "ladder" of the DNA molecule are always the same distance apart, making the molecule a constant width. This occurs because when the bases hydrogen bond to form the rungs of the "ladder", a:

a.	purine always pairs with a pyrimidine.

b.	purine always pairs with a purine.

c.	pyrimidine always pairs with a pyrmidine.

d.	The structure of the double helix is not determined by how the bases pair.

17. The RNA transcript of DNA that travels to the cytoplasm, carrying the instructions to make a protein, is called:

rRNA.

tRNA.

mRNA.

d.	RNA polymerase.

18. When DNA is replicated, the error rate is approximately one error for every 10,000 nucleotides copied. However, that error rate is reduced to only 1 error for every 1 billion nucleotides. The DNA sequence is "corrected" by:

a.	repeating S phase to see if the error rate is lower the second time around.

b.	enzymes that proofread the DNA and repair errors.

c.	messenger RNA during the process of transcription.

d.	Any errors made during DNA replication cannot be corrected. If errors are detected after S phase, the cell is destroyed.

19. Mendel's principle of segregation says that:

a.	when gametes are formed, each gamete receives only one allele for a particular gene.

b.	some genes are dominant to others.

c.	a testcross must be used to determine the genotype of an organism with a dominant phenotype.

d.	all of the above

20. Tay-Sachs disease is lethal before reproductive age, but the allele persists in Louisiana French Canadians because:

a.	it is a dominant allele.

b.	new mutations causing this disease are common in that population.

c.	it is not expressed in the phenotype of heterozygous individuals.

d.	the disease is contagious and can be transmitted by nonheritable means.

21. Physical features that are similar in organisms which are not closely related to each other are most likely a result of ____________. A good example of this is the similarities seen between some marsupial and placental mammals.

a.	natural selection.

b.	adaptation to similar environments

c.	convergent evolution

d.	All of the above phrases can be used to correctly fill this blank.

22. In humans, which of the following sex chromosome compliments could be found in males?

23. The proteins that are associated with eukaryotic chromosomes:

a.	help to package the DNA within the nucleus.

b.	are involved in gene expression, through binding with the DNA.

c.	may be replicating or transcribing the DNA.

d.	All of these are true of proteins that are associated with eukaryotic chromosomes.

24. A sequence of DNA nucleotides that contains the information to produce a single protein is a(n):

codon.

gene.

c.	polypeptide.

d.	anticodon.

whitelizard672

22. What is probably the single most important distinction between bacteria and eukaryotes?

a. transcription

b. the separation of the cell's genetic material from the surrounding cytoplasm

c. the existence of ribosomes

d. RNA processing in bacteria

e. antibiotics

23. Which one does NOT characterize an open reading frame (ORF)

a. Uses six reading frames during translation

b. Contains a start codon

c. Contains a stop codon

d. Its length is in multiples of 3 nucleotides

e. It should have the number of codons for a protein or on average ~250 codons in length.

24. Nested intronic genes lie within the non-coding intronic region of a larger gene, and occur in the human genome.

a. True

b. False

25. How would you characterize this statement: EF-Tu will hydrolyze GTP only if the codon:anticodon & tRNA:amino acid matchings are correct

a. Transcription; initiation steps

b. Transcription; termination steps

c. Translation: initiation steps

d. Tanslation: elongation steps

e. Spliceosome

26. Regarding the transcription unit architecture of the Escherichia coli genome, which one is FALSE?

a. Each of the 4,661 transcription units comprised an average of 1.1 modular units.

b. 3,010 transcription units (~65%) are monocistronic.

c. 1,652 transcription units (35%) contain more than one ORF (polycistronic)

d. The largest transcription unit is equivalent to 16 ORFs with numerous introns.

e. 398 transcription units (~9%) are nested within each other in opposite transcriptional orientation

27. Regarding RNA interference (RNAi), which one is FALSE?

a. It is a common and conserved mechanism in eukaryotic cells.

b. Originally discovered in bacteria and plant viruses.

c. A form of primitive immunity to protect the genome.

d. Pre microRNA (miRNA) genes have been found in introns.

e. Recent research shows that exogenous plant miRNAs in food can regulate the expression of target genes in mammals.

28. Aminoacyl-tRNA synthetases

a. Recognize the 3âUTR

b. The genes for these enzymes fall into the family of inducible genes

c. The enzymes are imported into the nucleus

d. Covalently attaches an amino acid to the appropriate tRNA. This takes place in the cytoplasm.

e. Forms the peptide bond during protein synthesis

29. What is the process by which complementary RNAs are made from a DNA template?

a. translation

b. transcription

c. protein synthesis

d. transduction

e. transliteration

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BIOL 212 Lecture Notes - Lecture 13: Reverse Transcriptase, Central Dogma Of Molecular Biology, Rna

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