BSCI 10001 Lecture Notes - Lecture 13: Light-Dependent Reactions, Photorespiration, Green Sulfur Bacteria

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So now lets take a closer look at how the calvin cycle uses atp and nadph (derived from the. Light reactions) to convert co2 to a sugar precursor via the dark reactions . In the 1950"s, melvin calvin and his co-workers at berkeley worked out the pathway whereby. Because 3-phosphoglycerate is labeled with 14c within seconds of adding 14co2 to the cells, Calvin and his team concluded that this 3-carbon compound is the initial product of the co2- fixation pathway. The investigators were initially thinking that co2 (a 1-carbon molecule) must 1st be reacted with some 2-carbon molecule to form that 3-carbon molecule, 3-phosphoglycerate. The key insight came when calvin realized it could combine with a 5-carbon molecule (ribulose bisphosphate) ( rubp ), to form a 6-carbon molecule that would then immediately split into two (3-carbon) molecules of 3-phosphoglycerate. The enzyme that catalyzes the condensation of co2 onto rubp is called rubp carboxylase/oxygenase (or rubisco ).

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