BIOL 150 Lecture Notes - Lecture 18: Mitochondrial Matrix, Intermembrane Space, Atp Synthase

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bronzefly406

15 Mar 2018

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Biol 150 principles of molecular & cellular biology. I stressed the ability of passage of electrons through the electron transport chain (etc) to force protons out of the mitochondrial matrix and into the intermembrane space, including an improvised demo with ta victoria (thanks, victoria!). Electrons attract protons as they are pulled from nadh to travel through the etc to oxygen. The enzyme complexes of the etc sometimes shield the electron charge from the protons so that the protons no longer follow the electrons and instead pass out of the membrane into the intermembrane space. As electrons travel from nadh to complex i to coenzyme q (quinone) to complex iii to cytochrome c to. Complex iv to oxygen, about 8 protons are pulled out of the mitochondrial matrix and shoved into the intermembrane space. The results are: 1) nadh is regenerated to. Fadh2 similar sends electrons to complex ii to coenzyme q to complex iii to.

Related Questions

1. Compared to the generation of ATP by oxidative phosphorylation, what is a major difference in mechanism of ATP generation by substrate-level phosphorylation in glycolysis?

A. A proton gradient is not involved

B. The ATP produced has less energy content because oxygen was not involved.

C. Substrate level phosphorylation occurs in the inter-mitochondrial space

D. One can't generalize without knowing the substrate.

2. Which of the following statements is true?

A. Because the electrons in NADH are at a higher energy than the electrons in reduced ubiquinone, the NADH dehydrogenase complex can pump more protons than can the cytochrome b-c1 complex.

B.The pH in the mitochondrial matrix is higher than the pH in the intermembrane space.

C. The proton concentration gradient and the membrane potential across the inner mitochondrial membrane tend to work against each other in driving protons from the intermembrane space into the matrix.

D. The difference in proton concentration across the inner mitochondrial membrane has a much larger effect than the membrane potential on the total proton-motive force.

2. The free energy of ATP synthesis from ATP synthase under cellular conditions is +45 kJ/mol.

a. Where does the energy come from?

b. If the pH of the matrix is 7.84, the intermembrane space is 7.16 and the membrane potential is ï±0.15 V at 37 Â°C, how much energy can be produced from this gradient per mol of protons?

c. How many moles of protons moving across this gradient would be required to produce enough energy for one mole of ATP to be synthesized?

d. To generate a proton gradient the mitochondria use the energy of electron transport. How much energy is produced in the oxidation of NADH by oxygen (under standard biochemical conditions)?

e. Using your answer from b, how many moles of protons could be transported out of the matrix with this oxidation energy (d)?

f. How many moles of protons are actually transported in the oxidation of NADH through the ETC?

magentajellyfish891

The free energy of ATP synthesis from ATP synthase under cellular conditions is +45 kJ/mol.

a. Where does the energy come from?

b. If the pH of the matrix is 7.84, the intermembrane space is 7.16 and the membrane potential is +/- 0.15 V at 37 C, how much energy can be produced from this gradient per mol of protons?

c. How many moles of protons moving across this gradient would be required to produce enough energy for one mole of ATP to be synthesized?

d. To generate a proton gradient the mitochondria use the energy of electron transport. How much energy is produced in the oxidation of NADH by oxygen (under standard biochemical conditions)?

e. Using the answer from b, how many moles of protons could be transported out of the matrix with this oxygen energy (d)?

f. How many moles of protons are actually transported in the oxidation of NADH theough the ETC?

bronzegoat54

BIOL 150 Lecture Notes - Lecture 18: Mitochondrial Matrix, Intermembrane Space, Atp Synthase

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