POL SCI 2 Lecture Notes - Lecture 17: Dynein, Ascl1, Pax6
Document Summary
Part 1: neurogenesis: spatial and temporal mechanisms decide the identity of the cell. Different neurons are made across the dorsal-ventral axis (d-v axis). This is decided according to levels of morphogenic gradients from the roof-and floorplate; lateral bmp and shh respectively as they give positional information. This implies that specific proneural genes are activated across the d-v axis. Pax7 activates ascl1 in the dorsal progenitor domain while pax6 and nkx6. 1 activate neurog1 in ventral progenitor domains: proneural genes work in combination with patterning genes to trigger differentiation of a specific neuron. This is not only found in spinal cord, but also in the retina. Here various homeodomain tfs combine with bhlh tfs to form unique combinations and give rise to a specific cell type: this can be replicated in vitro experiments. Es cells can be cultured and exposed to various tfs need to make a specific cell type, such dopaminergic neurons using.
