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Lecture 12

BIOL 1201 Lecture Notes - Lecture 12: Thylakoid, Electron Transport Chain, Water SplittingPremium


Department
Biological Sciences
Course Code
BIOL 1201
Professor
Moroney, James
Lecture
12

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BIOL 1201- Lecture – Photosynthesis/
Light Absorption
Growth of bacteria is greatest at red and blue wavelengths
Photosynthetic rate is highest at these wavelengths
Photosynthesis depends on chlorophyll
Light Reactions
Light energy absorbed by pigments in two protein–pigment complexes: Photosystems I
& II
The photostems, chlorophyll and electron transport chain are embedded in thylakoid
membranes
Light energy drives electron transport (E.T.) from H2O to NADP+
Electron transport produces H+ gradient across thylakoid membrane
ATP is formed by chemiosmosis
Double bonds- molecule that absorbs visible light
Photosystems:
oWhere
Water splitting reaction done by photosystem II
Where does oxygen come from, water.
Light energy drives electron transport (E.T.) from H2O to NADH
Electron transport produces H+ gradient across thylakoid membrane
ATP is formed by chemiosmosis
Calvin Cycle
NADPH and ATP from the light reactions are used to fix CO2 into sugars
There are three phases: Carbon Fixation, Reduction and Regeneration
Ribulose bisphosphate carboxylase/ oxygenase (rubisco) enzyme catalyzes Carbon
Fixation
Triose phosphate is the direct product
Will have to balance equations
oChapter 9 you will have to balance carbons- glycolysis
New G3P
Glyceraldehyde 3-phosphate (triose phosphate)
oAccumulation in the chloroplast leads to starch synthesis (glucose polymer)
oTransport into the cytoplasm
Provide energy via glycolysis
Used for synthesis of sucrose; transported through out the cell
Rubisco
Most abundant protein on the earth!
Catalyzes two reactions in C3 plants:
3 (P-5C-P) + 3 CO2 à 6 (3C-P) “good”
High [CO2] favors “good” reaction
Hot, dry conditions reduces leaf [CO2], favors “bad” reaction
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