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Lecture 14

BIOL 1201 Lecture Notes - Lecture 14: Pyrimidine Dimer, Dna Polymerase, ProofreadingPremium


Department
Biological Sciences
Course Code
BIOL 1201
Professor
Moroney, James
Lecture
14

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BIOL1201Lecture14DNARepair,TranscriptionandTranslation
DNARepair
● 
o LigaselinksthenewlymadeDNAfragments
o HelicaseunwindstheDNA
o PrimaseaddsasmallRNAprimertohelpDNApolymerace
o DNApolymeraceIIIbuildsmostofthenewDNAstrand
o DnapolymeraseIeditsouttheRNAprimer,fillsinthegapwithRNA
● DNAPolymerase“proofreads”newlyreplicatedDNA
● Otherenzymesalsoproofread
● Normalmismatchoccursonein10,000basepairs
● Proofreadingreduceserrortoonein1billionbasepairs
o UVlightcreatesthyminedimerandifnotproofreaditturnsintoskincancer
● DamagedDNAcanberepairedbynucleases
● Fig.16.16
**ClickerQuestions**
● ATPisaNucleotide
● WhenHersheyandChaselabeledbacteriophafewith35Swhatdidheylabel?
Protein
● WhenHersheyandChaselabeledbacteriophagewith32Pwhatdidtheylabel?
Nucleotide
● Dnareplicationissemiconservative
● Proteinfromdeadstreptococcuspneumonia“S”isaddedtoliving“R”cellsand
injectedintomice.Whathappens?MicelivedependsonDNAnotprotein
● 
Chapter17
Transcription/Translation
● GeneregionoftheDNAthatcodesforanRNAoranmRNAthatmakeaprotein
GeneticCode
● Geneticcodemustgivesequenceofaminoacidsfromsequenceofnitrogenousbases
● Thereare20aminoacidsinnaturalproteins
● Howmanybasesareneededtocodeforanaminoacid?3
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