BIOL 1208 Lecture Notes - Lecture 5: Paper Chromatography, Elution, Tae Buffer
Document Summary
To release click plunger all the way: mechanical/mircopipette: used for small volume transfers (less than 1ml), volumetric: designed to deliver a single volume precisely. The most accurate, but: mohr: cheap to make and least accurate. Marking at tip is maximum volume: serological: many gradations, not as accurate as volumetric, but more accurate there is only one measurement. than mohr. Spectrophotometer: turn it on (left hand knob on the front of the instrument) and allow to warm up for 15 minutes, adjust the wavelength to the appropriate value. If not, push mode button: carefully insert the tube containing the blank solution into the sample holder (after using kimwipe) and close the cover. It is used as a dna size marker: agarose gel: stationary phase, electric field: mobile phase, separates charged molecules using an electric field. It"s a matrix, allows larger molecules to move slower than smaller molecules. Tubes are inverted into substrate and allowed to sit.
