BIOL 4190 Lecture Notes - Lecture 1: Internal Ribosome Entry Site, Viroplasm, Eukaryotic Small Ribosomal Subunit (40S)
Document Summary
Genome 10-12 segments (11 for rotavirus) of dsrna (18-32 kpb). Virion icosahedral symmetery; naked; spokes of a wheel by vp6; spikes by vp4. Causes gastroenteritis in humans and animals. (stomach flu, diaherra) Rdrp; yes, it is located in the core; one of the largest vps. Discrete regions in the cytoplasm where rotavirus proteins accumulate. Nsp2 and nsp5 play a role in the formation of viroplasms. Virion cores are assembled in the viroplasms (from vp1, vp2, vp3). Yes, they form during the early events of replication. The double-layered particles are synthesized in the cytoplasm (viroplasm). The outer layer (vp7) and the spikes (vp4) are assembled in the rough endoplasmic reticulum. Vp7 and nsp4 synthesized in rough er where they remain localized in the membrane. Nsp4 has binding sites for vp4 and double-layered particles. After binding these components, the immature virion buds through the membrane into a vesicle and this forms the temporary membrane.
