BIO_SC 2200 Lecture Notes - Lecture 6: Thymine, Guanosine, Sigma Factor
9 Sep 2017
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One gene can use the top strand of the double helix, while another can use the bottom: dna template is read 3" 5". Rna is synthesized 5" 3": template strand = non-coding strand (used by rna pol). Coding strand = non- template strand (~ same seq as rna: the rna precursors are ribonucleoside triphosphates atp, gtp, ctp, and. They are: initiation: (rna pol) recognizes -35 and -10 regions, elongation: begins, released, core rna pol continues, termination: by p-dependent or -independent terminators, *elongation is conserved between prokaryotes and eukaryotes. Transcription in prokaryotes: a prokaryotic gene has three regions, a promoter sequence that attracts rna polymerase to begin transcription at a site specified by the promoter, the transcribed sequence is called the rna-coding sequence. 10 region (centered at -10bp: transcription initiation requires the rna polymerase holoenzyme (complete enzyme) to bind to the promoter. Core enzyme of rna polymerase (4 subunits, ")
