BIO 350 Lecture Notes - Lecture 12: Har Gobind Khorana, Prokaryotic Small Ribosomal Subunit, Transfer Rna
Document Summary
Translation: rna is the template for protein synthesis. Initiation: shine-delgarno, kozak, ifs and eifs, initiator aminoacyl-trna: elongation: codons, ef and eefs, pabp, peptidyl-trna, termination: amber, ochre, opal, release factors, deacylated-trna, uag ("amber", uaa ("ochre", uga ("opal") What is responsible for translation: ribosomes are large ribonucleoprotein particles that contain more rna than protein and dissociate into large and small subunits. How do ribosomes exist, what form do they take in a cell: ribosomes are only complete when engaged in translation, 70s (bacteria, 80s (eukaryotes, ribosomal subunits are available in the cell for assembly and translation. Robert w. holley, har gobind khorana and marshall w. nirenberg "for their interpretation of the genetic code and its function in protein synthesis". How do trnas interact with the ribosome: the ribosome has three trna-binding sites, peptidyl-trna occupies p site. Site contains trna with the growing protein attached: aminoacyl-trna enters a site. trna with amino acid attached, deacylated trna exits via e site.
