GN 311 Lecture Notes - Lecture 19: Reverse Transcriptase, Moodle, Psy
Document Summary
Creating new dna molecules combining dna from different sources. Reads the sa(cid:373)e 5" to 3" o(cid:374) either stra(cid:374)d for a seg(cid:373)e(cid:374)t of dna. Ligase is used in replication to ligate together the okazaki fragments and in dna repair to seal the nicks. Carrier dna molecule that is capable of independent replication into which a dna fragment can be cloned. Its purpose is to carry foreign dna into the cell. The best way to make dna is to give to bacteria to make a bunch of it. Plasmid dna is good because it is circular and dna is more stable this way. Also need a single cleavage site for each restriction enzyme. Allow cells containing vector and the recombinant molecule to be identified. Insertional marker to notify that there was dna in a sample which you are moving. Transform dna into bacteria (bacteria takes up the dna and makes more)
