BIOL 2301 Lecture Notes - Nematode, Cell Migration, Wild Type

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17 Feb 2014
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Feed on e. coli and live approximately 2-3 weeks. Ease of maintaining the population in the laboratory. Easy long-term storage because they remain viable after being frozen. It is multicellular and yet simple enough to be studied relatively easily: brenner. Anatomy is invariant from one organism to the next because of its size. More directed approach became useful as they got down to the nitty gritty. As these random-sampling-based methods become less efficient at gene confirmation/discovery, directed methods that begin with the predicted gene models became more useful. As part of an effort to obtain full-length cdna clones for all c. elegans genes (the orfeome project) (lamesch et al. 2004), >12,500 orfs have been cloned in gateway vectors, using rt-pcr starting from the gene models. Preliminary analysis of the data indicates that the gene set may rise to >21,000 confirmed protein-coding genes.

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