BIOL 2301 Lecture Notes - Potassium Acetate, Natural Transformation, Plasmid

70 views7 pages
17 Feb 2014
Department
Course
Professor

Document Summary

Transformation of e. coli and analysis of plasmid dna. The first exercise in this multi-step experiment was the transformation of e. coli with the plasmid pamp. The bacteria in the lab were transformed with plasmid dna containing an ampicillan resistance gene. In genetics, the natural transformation of bacteria is the incorporation of genetic material from a foreign source in the environment (begley 2007). In this experiment, two steps are needed in order to transform the bacteria. The first step is to prepare competent bacterial cells that can be used for transformation. The next step is the actual transformation of competent cells. This is done by adding the pamp solution to the competent cells. Six plates were prepared, 3 with ampicillan and 3 without ampicillan. The growth on these plates was observed and recorded. The second exercise was a mini-prep done to prepare plasmid dna.

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers
Class+
$8 USD/m
Billed $96 USD annually
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
30 Verified Answers

Related Documents

Related Questions