BIOL 2324 Lecture Notes - Chitinase, Glutamine, Molecular Mass

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Chitinase [homerus americanus] ikkgappakl vlgvpfygrs wtlagsdast gaaasgpgrp gplmkdsgsl ayyevclahe rdgwtkvssg eaphltsddq wvgyddidtv trkaqyalkk glggvmiwdi amddfrgscs agvnpllsai srtlrgasgl rltgppttvm ptrprpiipt hpepprsits rprpttaspt mqlnlaecrr leytadlvnc dhfyrcindk vfhfqcpkgl hwrqsrascd wpkaalckar svt. Please note the modified algorithm for extinction coefficient. Number of amino acids: 243 molecular weight: 26423. 2 theoretical pi: Total number of negatively charged residues (asp + glu): 21 total number of positively charged residues (arg + lys): 31 atomic composition: carbon c. 13 formula: c1163h1835n339o340s13 total number of atoms: 3690. Extinction coefficients: extinction coefficients are in units of m-1 cm-1, at 280 nm measured in water. Ext. coefficient 43930 abs 0. 1% (=1 g/l) 1. 663, assuming all pairs of cys residues form cystines. Ext. coefficient 43430 abs 0. 1% (=1 g/l) 1. 644, assuming all cys residues are reduced estimated half-life: the n-terminal of the sequence considered is i (ile). The estimated half-life is: 20 hours (mammalian reticulocytes, in vitro).

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