BIOL-UA 21 Lecture Notes - Lecture 24: Sanger Sequencing, Bacs, Reference Genome
18 Mar 2016
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Document Summary
Putting bacs in order with re mapping. Define a set of bac clones that spans genome with minimal redundancy. Minimal tiling path = with 5 bacs you can cover the whole region with minimal overlap! Select and subclone bacs into smaller pieces for sequencing. Place an amount of ~1000 bases and do sanger sequencing. Once sequences were determined, for each set of sequences they were. Hierarchical shotgun sequencing assembled using shotgun sequence. First give the chromosome number and then the coordinates that will tell you exactly where you are in the gnome (just like gps coordinates) After you sequence the genome you try to know the structure, and know where the genes are! Blue shoes you the resulting gene from synthesizing all available. After we"ve done all of this: we do not know the function of these proteins. Allows you to search for any gene on ucsc. Percentage of disease genes shared with humans.
