BIOL-UA 22 Lecture Notes - Lecture 2: Critical Micelle Concentration, Integral Membrane Protein, Peripheral Membrane Protein
18 Mar 2016
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Document Summary
Peripheral membrane proteins lipid-anchor protein = protein attaches to the lipid (covalently) and attach to the membrane. Biochemical challenge for integral membrane proteins: interacting with the hydrophobic core of the lipid bilayer: Protein backbone is very hydrophilic, meaning it usually doesn"t like to be in the membrane. If attached to different other chains this can allow it to have more hydrophobic character and help it travel across. If you want to embed protein in membrane, you choose amino acid that contains hydrophobic character inside the membrane avoid any polar charged amino acid. Serine & tyrosine both have -oh groups making them hydrophilic. Hydropathy proiles can predict transmembrane domains from protein sequences showing where the amino acid is hydrophobic or hydrophilic characteristic based on their position in the membrane. Changes in value indicate attraction of specific protein regions towards the hydrophobic region inside lipid bilayer. Amphipathic detergents are required to solubilize membrane proteins. Ionic denature proteins at high concentrations.
