BIOLOGY 1113 Lecture Notes - Lecture 43: Molecular Cloning, Cloning Vector, Recombinant Dna

• School: Ohio State University
• Department: Biology
• Course: BIOLOGY 1113
• Professor: Eric Danhart

Document Summary

All credit goes to freeman biology 6th edition 2017. Chapter 20: the molecular revolution: biotechnology and beyond. Genome- all hereditary material in virus, cell, organism, including but not only genes. Genomics- field of study concerned w/ sequencing, interpreting/comparing whole genomes of different organisms. Recombinant dna technology- variety of techniques for isolating specific dna fragments, liking dna to different regions of dna/adding them to different organism. Dna cloning- techniques producing many identical copies of particular gene/dna sequence. Plasmid- small, circular, supercoiled dna molecule independent of cell"s main chromosome in prokaryotes and some eukaryotes. Cloning vector- plasmid/agent(virus) used to transfer recombinant genes into cultured host cells. Add gene to plasmid, force bacteria to take up plasmid from enviroment, produce and harvest product. Dna at specific base-pair sequence(recognition site)(used as defense against viruses). In order to insert human gene into plasmid, both must be cut by same restriction enzyme.